diff --git a/packages/google-cloud-spanner/noxfile.py b/packages/google-cloud-spanner/noxfile.py
index 39c7c32036b4..e1a13b2ea098 100644
--- a/packages/google-cloud-spanner/noxfile.py
+++ b/packages/google-cloud-spanner/noxfile.py
@@ -31,7 +31,6 @@
 SYSTEM_TEST_PYTHON_VERSIONS: List[str] = ["3.12"]
 
 ALL_PYTHON: List[str] = [
-    "3.9",
     "3.10",
     "3.11",
     "3.12",
@@ -71,7 +70,6 @@
 CURRENT_DIRECTORY = pathlib.Path(__file__).parent.absolute()
 
 nox.options.sessions = [
-    "unit-3.9",
     "unit-3.10",
     "unit-3.11",
     "unit-3.12",
diff --git a/packages/google-cloud-spanner/setup.py b/packages/google-cloud-spanner/setup.py
index 6c076979fbe7..babf000c3928 100644
--- a/packages/google-cloud-spanner/setup.py
+++ b/packages/google-cloud-spanner/setup.py
@@ -86,7 +86,6 @@
         "License :: OSI Approved :: Apache Software License",
         "Programming Language :: Python",
         "Programming Language :: Python :: 3",
-        "Programming Language :: Python :: 3.9",
         "Programming Language :: Python :: 3.10",
         "Programming Language :: Python :: 3.11",
         "Programming Language :: Python :: 3.12",
@@ -97,7 +96,7 @@
     ],
     platforms="Posix; MacOS X; Windows",
     packages=packages,
-    python_requires=">=3.9",
+    python_requires=">=3.10",
     install_requires=dependencies,
     extras_require=extras,
     include_package_data=True,
diff --git a/packages/google-cloud-spanner/testing/constraints-3.9.txt b/packages/google-cloud-spanner/testing/constraints-3.9.txt
deleted file mode 100644
index ac3833d41b9a..000000000000
--- a/packages/google-cloud-spanner/testing/constraints-3.9.txt
+++ /dev/null
@@ -1,13 +0,0 @@
-# -*- coding: utf-8 -*-
-# This constraints file is used to check that lower bounds
-# are correct in setup.py
-# List all library dependencies and extras in this file,
-# pinning their versions to their lower bounds.
-# For example, if setup.py has "google-cloud-foo >= 1.14.0, < 2.0.0",
-# then this file should have google-cloud-foo==1.14.0
-google-api-core==2.21.0
-google-auth==2.35.0
-# TODO(https://github.com/googleapis/gapic-generator-python/issues/2453)
-# Add the minimum supported version of grpcio to constraints files
-proto-plus==1.22.3
-protobuf==4.25.8